Related Experiment Video
Updated: Apr 20, 2026

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange ABE
Published on: February 18, 2013
Identification of the WNT1 residues required for palmitoylation by Porcupine
M Miranda1, L M Galli1, M Enriquez1
1Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.
Abstract:
The post-translational palmitoylation of WNT morphogens is critical for proper signaling during embryogenesis and adult homeostasis. The addition of palmitoyl groups to WNT proteins is catalyzed by Porcupine (PORCN). However, the Wnt amino acid residues required for recognition and palmitoylation by PORCN have not been fully characterized. We show that WNT1 residues 214-234 are sufficient for PORCN-dependent palmitoylation of Ser224. Substitution of Ser224 with Thr, but not Cys, is tolerated in palmitoylation and biological assays. Our data highlight the importance of palmitoylation for WNT1 activity and establish PORCN as an O-acyl transferase for WNT1.
Related Concept Videos
Non-Canonical Wnt Signaling Pathways
Canonical Wnt Signaling Pathway
Canonical Wnt Signaling Pathway
Lipids as Anchors
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
Structure of Porins

