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Updated: Apr 20, 2026
Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Studies on cytotoxic pregnane sapogenins from Cynanchum wilfordii
Lie-Jun Huang1, Bing Wang2, Jian-Xin Zhang3
1Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, PR China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, PR China.
Abstract:
To investigate their cytotoxicity, seventeen C21-steroidal pregnane sapogenins 1-17 were isolated from the hydrolytic extracts of the roots of Cynanchum wilfordii. Among them, sapogenins 1-7 are new compounds, whose structures were determined by extensive analysis of spectroscopic data and X-ray crystallographic analysis. Especially, sapogenins with salicyl or vanilloyl group, and a new aberrant pregnane skeleton with ether linkage between C-12 and C-20 were found for the first time. Compound 1 revealed significant cytotoxicities on HL-60 (IC50 6.72μM) and MCF-7 (IC50 2.89μM), and compounds 14 and 15 also revealed strong inhibitory activities against K-562 (IC50 6.72μM) or MCF-7 (IC50 2.49μM), respectively.
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