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Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
Published on: January 3, 2017
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Connections matter: channeled hydrogels to improve vascularization.
Severin Muehleder1, Aleksandr Ovsianikov2, Johannes Zipperle1
1Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center , Vienna , Austria ; Austrian Cluster for Tissue Regeneration , Vienna , Austria.
Frontiers in Bioengineering and Biotechnology
|December 3, 2014
Summary
Fabricating hollow channels in cell-laden hydrogels is crucial for soft tissue engineering. This strategy enhances vascularization, improving cell survival and implant function by increasing oxygen and nutrient supply.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Cell-laden hydrogels are emerging for soft tissue engineering.
- Vascularization of engineered constructs is critical but often underestimated.
- Lack of vascularization leads to cell death, impaired function, and implant failure.
Purpose of the Study:
- To discuss the fabrication of hollow channels within hydrogels as a strategy to facilitate vascularization.
- To review methods for creating channels in hydrogels.
- To highlight the importance of endothelial cells for in vivo applications.
Main Methods:
- Fabrication of hollow channels using removable spacers.
- Application of 3D laser processing strategies.
- Utilizing planar processing strategies for channel creation.
Main Results:
- Hollow channels promote controlled cell distribution.
- Engineered channels increase oxygen transport and nutrient supply in vitro.
- This approach enhances the potential for vascularization in engineered tissues.
Conclusions:
- Hollow channels are a promising strategy for improving vascularization in hydrogel-based tissue engineering.
- Incorporating endothelial cells is vital for promoting vessel ingrowth and anastomosis.
- Careful selection of endothelial cell types is necessary for successful in vivo translation.
