Single molecule microscopy in 3D cell cultures and tissues.

Area of Science:

• Cell Biology
• Microscopy

Background:

• Single molecule microscopy provides unparalleled insight into cellular dynamics.
• Historically, its application has been limited to 2D cell cultures, excluding 3D models.

Purpose of the Study:

• To review recent progress enabling single molecule microscopy in 3D cell systems.
• To highlight technical advancements facilitating this expansion.

Main Methods:

• Review of technical innovations in single molecule microscopy.
• Adaptation of microscopy techniques for 3D cell culture environments.

Main Results:

• Single molecule microscopy can now be applied to complex 3D cell models.
• Technical challenges have been overcome, allowing for detailed molecular insights in 3D.

Conclusions:

• Single molecule microscopy in 3D cell models is now feasible.
• 3D cell models will become integral to the future success of single molecule microscopy.