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Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
Published on: July 16, 2021
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Topographical cues regulate the crosstalk between MSCs and macrophages.
Gema Vallés1, Fátima Bensiamar1, Lara Crespo1
1Hospital Universitario La Paz-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, Spain; Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Spain.
Biomaterials
|December 3, 2014
Summary
Scaffold topography influences mesenchymal stem cells (MSCs) and macrophage communication. Three-dimensional (3D) structures promote anti-inflammatory responses and reduce inflammatory factors, impacting cell migration.
Area of Science:
- Biomaterials Science
- Immunology
- Cell Biology
Background:
- Scaffold implantation can trigger foreign body responses mediated by macrophages.
- Surface topography of biomaterial scaffolds influences mesenchymal stem cell (MSC) behavior and function.
Purpose of the Study:
- To investigate if surface topographical features of scaffolds can modulate paracrine interactions between MSCs and macrophages.
- To understand how 3D scaffold architecture affects MSC-macrophage communication and inflammatory signaling.
Main Methods:
- Co-culture of MSCs and macrophages on 2D versus 3D scaffold topographies.
- Measurement of secreted inflammatory and anti-inflammatory proteins (PGE2, TSG-6, IL-6, MCP-1).
- Analysis of mRNA levels and use of neutralizing antibodies to elucidate signaling pathways.
Main Results:
- 3D scaffold topography induced MSCs to produce anti-inflammatory proteins PGE2 and TSG-6.
- 3D co-cultures significantly reduced secretion of pro-inflammatory factors IL-6 and MCP-1 compared to 2D.
- Reduced MCP-1 mRNA levels in 3D co-cultures correlated with decreased secretion.
- 3D-arranged MSCs created an inflammatory milieu that decreased monocyte migration.
Conclusions:
- Scaffold surface topography is critical for regulating MSC-macrophage paracrine interactions.
- 3D micro-architectures promote anti-inflammatory signaling and modulate immune cell responses.
- Topographical cues guide soluble factor-mediated communication, influencing the local inflammatory environment.
