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Updated: Apr 19, 2026
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
Published on: February 7, 2025
Comparative secretomic and N-glycoproteomic profiling in human MCF-7 breast cancer and HMEpC normal epithelial cell
Aik-Aun Tan1, Alan Kang-Wai Mu2, Lik-Voon Kiew3
1Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, 11800 Malaysia.
Background:
Concurrent study of secretomic and glycoproteomic profiles in cancer cell lines represents an excellent approach for investigating cancer progression and identifying novel biomarker candidates. In this study, we performed a comparative secretomic and N-glycoprotein profiling from the secretions of normal human mammary epithelial cells (HMEpC) and the MCF-7 human breast cancer cell line.
Method:
We analyzed these cell lines using a combined methodology involving glycan-binding lectins and two-dimensional electrophoresis and identified several differentially secreted factors, including osteonectin and haptoglobin.
Result:
Notably, comparative analyses also revealed that MCF-7 cells produced differentially N-glycosylated forms of haptoglobin.
Conclusion:
The present data suggested that osteonectin and haptoglobin might have potential to be served as potential biomarkers for breast cancer. However, further investigation is needed to validate the findings.
