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Microfluidic Chip Fabrication and Method to Detect Influenza

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Published on: March 26, 2013

Droplet microfluidics for chip-based diagnostics.

Karan V I S Kaler¹, Ravi Prakash²

¹Department of Electrical and Computer Engineering, University of Calgary, 2500 University Drive NW, Calgary, AB-T2N 1N4, Canada. kaler@ucalgary.ca.

Sensors (Basel, Switzerland)

|December 10, 2014

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Electro-actuation droplet microfluidics (DMF) precisely manipulates tiny fluid volumes on-chip. This review covers electro-actuation DMF devices for lab and clinical diagnostics.

Area of Science:

Microfluidics
Biotechnology
Nanotechnology

Background:

Droplet microfluidics (DMF) offers precise control over micro- to picoliter volumes.
Various droplet actuation methods exist, using external stimuli for manipulation.
Electro-actuation DMF utilizes patterned substrates and electric fields for sample handling.

Purpose of the Study:

To review the operation and utility of electro-actuation-based DMF devices.
To discuss the theory, fabrication, and detector integration in DMF.
To illustrate applications in laboratory and clinical diagnostics.

Main Methods:

Focus on electro-actuation methods within droplet microfluidics.
Review of device fabrication and integration of optical/opto-electronic detectors.

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Discussion of underlying theory for electric field-based droplet manipulation.

Main Results:

Electro-actuation DMF enables precise handling of chemical and biological samples.
Micro-/nano-patterned substrates are key for electric field-based control.
Integration of detectors enhances DMF capabilities for assays.

Conclusions:

Electro-actuation DMF is a versatile technology for miniaturized assays.
The review highlights the utility of DMF in chip-based diagnostics.
Further applications in laboratory and clinical settings are demonstrated.