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[Genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats]
Yun Wang1, Zhangjian Chen1, Te Ba1
1Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University Health Science Center, Beijing 100191, China.
Objective:
To explore the genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.
Methods:
Twenty-eight SD male young rats (4 weeks old) were randomly divided into 4 groups, which were exposed to TiO2 nanoparticles ((75 ± 15) nm, anatase) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight (bw) every day for 30 days. The bone marrow cells were collected for micronuclei and γ-H2AX immunofluorescence analysis.
Results:
The percentage of γ-H2AX foci-positive cells (37.4 ± 10.0)% in the 50 mg/kg bw dose group were significantly higher than that in the control group (19.8 ± 3.1)% (t value was -17.59, P < 0.01). No significantly difference was found in polychromatic erythrocyte/normochromatic erythrocyte (PCE/NCE) ratio and PCE micronucleus rate between three experimental groups and control group.
Conclusion:
TiO2 nanoparticles can increase the frequency of DNA double-strand breaks in bone marrow cells, but has no effect on micronucleus of bone marrow cells in young rats.
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