Related Experiment Video
Updated: Apr 19, 2026
11:27
X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
Published on: May 13, 2020
4.5K
Using the MEROPS Database for Proteolytic Enzymes and Their Inhibitors and Substrates
Neil D Rawlings1,2, Alan J Barrett1, Alex Bateman2
1Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
Current Protocols in Bioinformatics
|December 16, 2014
Summary
MEROPS is a comprehensive database for proteolytic enzymes, their inhibitors, and substrates. It provides detailed annotation, alignments, and interaction data, accessible online and locally.
Area of Science:
- Biochemistry
- Bioinformatics
- Proteomics
Background:
- Proteolytic enzymes, inhibitors, and substrates play crucial roles in biological processes.
- Understanding these interactions is vital for numerous research areas.
- Existing databases may lack comprehensive, integrated information.
Purpose of the Study:
- To present the MEROPS database, a resource for proteolytic enzymes and their inhibitors.
- To detail the organization of data within MEROPS, including families and clans.
- To provide guidance on accessing and utilizing MEROPS data.
Main Methods:
- MEROPS organizes proteolytic enzymes and protein inhibitors into protein domain families and clans.
- Each entry includes annotation, multiple sequence alignment, phylogenetic trees, and literature references.
- Interactions between enzymes, inhibitors, and substrates are cataloged.
Main Results:
- MEROPS offers a structured repository of information on proteolysis.
- Data is accessible through the World Wide Web, with options for remote and local use.
- Comprehensive details on accessing and utilizing the database are provided.
Conclusions:
- MEROPS serves as a valuable, integrated resource for researchers studying proteolysis.
- The database facilitates the exploration of enzyme-inhibitor and enzyme-substrate interactions.
- Easy access and utilization enhance its utility for the scientific community.
More Related Videos
Related Concept Videos
01:30Enzyme Inhibition
95.4K
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
95.4K
01:23Role of Matrix Metalloproteases in Degradation of ECM
3.8K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.8K
02:34Enzymes
97.7K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
97.7K
01:11Restriction Enzymes
40.3K
Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
40.3K
01:17The Proteasome Structure
2.3K
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
The proteasome is an...
2.3K