A device for dried blood microsampling in quantitative bioanalysis: overcoming the issues associated blood hematocrit
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Published on: January 13, 2023
Neil Spooner1, Philip Denniff, Luc Michielsen
1Bioanalytical Science & Toxicokinetics, Drug Metabolism & Pharmacokinetics, GlaxoSmithKline Research & Development, Ware, UK.
Aims:
A cross-laboratory experiment has been performed on a novel dried blood sampler in order to investigate whether it overcomes issues associated with blood volume and hematocrit (HCT) that are observed when taking a subpunch from dried blood spot samples.
Materials & Methods:
An average blood volume of 10.6 μl was absorbed by the samplers across the different HCTs investigated (20-65%).
Results:
No notable change of volume absorbed was noted across the HCT range. Furthermore, the variation in blood sample volumes across six different laboratories was within acceptable limits.
Conclusion:
The novel volumetric absorptive microsampling device has the potential to deliver the advantages of dried blood spot sampling while overcoming some of the issues associated with the technology.
