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ICECAP: an integrated, general-purpose, automation-assisted IC50/EC50 assay platform
Ming Li1, Judy Chou2, Kristopher W King2
1Biogen Idec, Inc., Cambridge, MA, USA ming.li@biogenidec.com.
Journal of Laboratory Automation
|December 20, 2014
Summary
A new automation-assisted platform streamlines drug potency assays (IC50/EC50). This system enhances experimental productivity and data processing efficiency for bioanalytical labs.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Pharmacology
Background:
- IC50 and EC50 values are crucial for assessing drug potency.
- Manual IC50/EC50 assays in mass spectrometry (MS)-based labs are time-consuming and prone to errors.
- Current automation solutions lack the throughput for IC50/EC50 assay demands.
Purpose of the Study:
- To develop an automated platform for IC50/EC50 assays.
- To address the limitations of manual and existing automated assay preparation methods.
Main Methods:
- Developed a general-purpose, automation-assisted platform for IC50/EC50 assays.
- Automated calculations for spiking and matrix solutions preparation.
- Integrated automated sample preparation, data extraction, and analysis.
Main Results:
- The platform automates solution preparation, sample extraction, data processing, and reporting.
- It handles calculations, nonlinear regression, curve fitting, and IC50/EC50 value computation.
- The system processes a wide range of assay conditions and concentrations.
Conclusions:
- The automation-assisted platform significantly improves experimental productivity and data processing efficiency for IC50/EC50 assays.
- It can process up to 32 compounds with 10 concentration levels per compound per run.
- This technology is applicable to the entire class of IC50/EC50 assays.
