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Efficient cell-specific uptake of binding proteins into the cytoplasm through engineered modular transport systems

Wouter P R Verdurmen1, Manuel Luginbühl1, Annemarie Honegger1

  • 1Dept of Biochemistry, University of Zurich, Winterthurerstr. 190, 8057 Zurich, Switzerland.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|December 21, 2014
PubMed
Summary

Researchers developed a new assay to measure cytosolic delivery of proteins. They created modular transport systems using DARPins and bacterial toxins, successfully delivering proteins into the cytosol via anthrax pores and Pseudomonas exotoxin A.

Keywords:
Anthrax toxinBiotin ligaseCytoplasmic deliveryDesigned ankyrin repeat proteinProtein engineeringPseudomonas exotoxin A

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