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Highly sensitive and selective detection of miRNA: DNase I-assisted target recycling using DNA probes protected by

Yi Xie1, Xiaoyan Lin, Yishun Huang

  • 1MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China. cyyang@xmu.edu.cn ljzhou@xmu.edu.cn.

Chemical Communications (Cambridge, England)
|January 3, 2015
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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...