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Updated: Apr 18, 2026

Introduction to Solid Supported Membrane Based Electrophysiology
Published on: May 11, 2013
Relaxation and Simplex mathematical algorithms applied to the study of steady-state electrochemical responses of
V Flexer1, K F E Pratt2, F Garay3
1INQUIMAE, Departamento de Quimica Inorganica, Analitica y Quimica Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Abstract:
A description of the implementation of the relaxation method with automatic mesh point allocation for immobilized enzyme electrodes is presented. The advantages of this method for the solution of coupled reaction-diffusion problems are discussed. The relaxation numerical simulation technique is combined with the Simplex fitting algorithm to extract kinetic parameters from experimental data. The results of the simulations are compared to experimental data from self-assembled multilayered electrodes comprised of glucose oxidase (GOx) and an Os modified redox mediator and found to be in excellent agreement.
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