High-density optrodes for multi-scale electrophysiology and optogenetic stimulation

Area of Science:

• Neuroscience
• Biomedical Engineering
• Materials Science

Background:

• High-resolution neural recording and stimulation are crucial for understanding brain function.
• Existing technologies often face limitations in invasiveness, tethering forces, and integrated functionality.

Purpose of the Study:

• To design and implement hybrid optical-electrical probes (optrodes) for simultaneous high-resolution electrophysiology and optogenetic stimulation.
• To develop a minimally invasive, scalable, and high-throughput system for neural interfacing in freely-behaving animals.

Main Methods:

• Fabrication of 64-channel, 50 μm × 20 μm implantable optrodes with a 1-2 mm span.
• Integration of a monolithic, high-density flexible cable (6 μm thin) and a lightweight 256-channel headstage (1.3 gr).
• Incorporation of a polymer-based multi-channel photonic light delivery system for localized optogenetic stimulation.

Main Results:

• Demonstrated successful design and implementation of hybrid optrodes.
• Achieved minimally invasive neural interfacing with reduced tethering forces.
• Developed a scalable manufacturing process for high-yield, high-throughput production.

Conclusions:

• The developed hybrid optrodes offer a versatile platform for advanced neuroscience research.
• The system facilitates high-resolution neural recording and precise optogenetic manipulation in multiple brain areas.
• The scalable design supports widespread adoption in preclinical research settings.