Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.2K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.3K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

15.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Portable <i>in vivo</i> confocal ophthalmoscope for non-contact imaging of the cornea and anterior segment of the eye.

Biomedical optics express·2025
Same author

Compact scattering-based light sheet microscopy probe using a custom miniature objective lens.

Journal of optical microsystems·2025
Same author

Introduction to the Biophotonics Congress 2024 feature issue.

Biomedical optics express·2025
Same author

An In Situ Curing, Shear-Responsive Biomaterial Designed for Durable Embolization of Microvasculature.

Advanced healthcare materials·2025
Same author

Point-of-need diagnostics in a post-Covid world: an opportunity for paper-based microfluidics to serve during syndemics.

Lab on a chip·2025
Same author

Dual-Mode Stretchable Emitter with Programmable Emissivity and Air Permeability.

ACS applied materials & interfaces·2024

Related Experiment Video

Updated: Apr 18, 2026

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

28.8K

Miniature objective lens with variable focus for confocal endomicroscopy.

Minkyu Kim1, DongKyun Kang2, Tao Wu2

  • 1Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA ; School of Engineering, The University of Tokyo, Yayoi 2-11-16 Bunkyo, Tokyo 113-8656, Japan.

Biomedical Optics Express
|January 10, 2015
PubMed
Summary

Researchers developed a miniature vari-focal objective lens for spectrally encoded confocal microscopy (SECM) to maintain consistent imaging depth in vivo. This innovation overcomes challenges posed by patient motion and tissue irregularities, enabling clearer visualization of cellular structures.

Keywords:
(170.1790) Confocal microscopy(170.2150) Endoscopic imaging(170.2680) Gastrointestinal(170.3890) Medical optics instrumentation(170.4730) Optical pathology(220.3620) Lens system design

More Related Videos

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

35.3K
Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

3.0K

Related Experiment Videos

Last Updated: Apr 18, 2026

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

28.8K
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

35.3K
Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

3.0K

Area of Science:

  • Biomedical Optics
  • Microscopy Technology
  • Endoscopic Imaging

Background:

  • Spectrally encoded confocal microscopy (SECM) enables rapid, high-resolution imaging of luminal organs.
  • Maintaining consistent imaging depth during in vivo SECM is challenging due to patient motion and tissue surface variations.

Purpose of the Study:

  • To develop a miniature vari-focal objective lens for SECM endoscopic probes.
  • To enable adaptive focusing and maintain constant imaging depth during in vivo SECM.

Main Methods:

  • Designed a vari-focal objective lens (5 mm diameter, 4 mm thickness) with an aspheric singlet (NA 0.5), miniature water chamber, and elastic membrane.
  • Adjusted water volume to alter membrane curvature, thereby controlling the SECM focus position.
  • Achieved a vari-focal range of 240 μm with lateral resolution < 2.6 μm and axial resolution < 26 μm.

Main Results:

  • Demonstrated adaptive focusing capability for SECM endoscopic probes.
  • Successfully maintained imaging depth across a 260 μm vari-focal range in swine esophageal tissue.
  • Acquired volumetric SECM images with clear visualization of cellular features like nuclei, papillae, and lamina propria.

Conclusions:

  • The developed miniature vari-focal objective lens effectively addresses in vivo depth-of-focus challenges in SECM.
  • This technology enhances the reliability and clarity of endoscopic SECM imaging for luminal organs.
  • Enables detailed cellular-level visualization of tissue structures, improving diagnostic potential.