Development of Na, K-ATPase in neocortical grafts

Pieces of cerebral cortex from 14-day rat embryos were transplanted into freshly prepared cavities in the cerebral cortex of adult rats. At various time intervals after implantation. Na,K-ATPase, Mg-ATPase as well as the ratio of two molecular forms of Na,K-ATPase were determined in the grafts and compared with the values obtained from intact cortex at various stages of postnatal development. Up to the 57th postimplantation day (PID), Na.K-ATPase develops very slowly, reaching on PID 57, i.e. on postconceptional day (PCD) 71, only 40% of the enzyme activity of intact brain cortex (PND 49, PCD 71). At PID 90 (PCD 104) Na,K-ATPase activity attained that of the intact adult tissue (PND 82, PCD 104). Mg-ATPase activity in the grafts developed similarly to that of intact brain but was much higher in the early postimplantation phase. The maximum ratio of the two molecular forms of Na,K-ATPase [alfa(+)/alfa] was shifted toward later developmental periods in the grafts as compared with the intact cerebral cortex.