Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-III01:22

Bioreactor Controls-III

59
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
59
Methods of Medium Optimization01:28

Methods of Medium Optimization

63
Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...
63
Scale-Up Processes01:14

Scale-Up Processes

92
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
92
Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

68
Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
68
Upstream Processing01:27

Upstream Processing

92
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
92
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

1.2K
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted versus Universally Offered Screening in Pediatric Emergency Departments: Cost-Effectiveness.

MDM policy & practice·2026
Same author

An Ounce of Prevention and a Pound of Cardiac Rehabilitation.

The Canadian journal of cardiology·2026
Same author

Hypertensive responses to exercise after 12-weeks of high-intensity interval training or moderate-to-vigorous intensity continuous training in patients with persistent and permanent atrial fibrillation: a non-prespecified post-hoc analysis of a randomized clinical trial.

Journal of science and medicine in sport·2026
Same author

A Glass Half-Full: Physical Activity Discussions Between Health Care Professionals and Patients With Atrial Fibrillation.

The Canadian journal of cardiology·2026
Same author

Reply to Soliman et al, Rapid HCV diagnostics: aligning molecular innovation with real-world elimination needs.

The Journal of infectious diseases·2026
Same author

Combined strength and aerobic training vs. aerobic training alone in patients with heart failure: A systematic review and meta-analysis.

Journal of sport and health science·2026
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Related Experiment Video

Updated: Apr 18, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.9K

Computational methods in metabolic engineering for strain design.

Matthew R Long1, Wai Kit Ong2, Jennifer L Reed2

  • 1Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, United States.

Current Opinion in Biotechnology
|January 11, 2015
PubMed
Summary
This summary is machine-generated.

Metabolic engineering employs computational tools to design microbial hosts for producing valuable compounds. These advanced methods identify novel biological pathways and genetic modifications for optimized chemical synthesis.

More Related Videos

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

4.4K
The Use of Chemostats in Microbial Systems Biology
13:19

The Use of Chemostats in Microbial Systems Biology

Published on: October 14, 2013

32.0K

Related Experiment Videos

Last Updated: Apr 18, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.9K
High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

4.4K
The Use of Chemostats in Microbial Systems Biology
13:19

The Use of Chemostats in Microbial Systems Biology

Published on: October 14, 2013

32.0K

Area of Science:

  • Biotechnology and metabolic engineering
  • Synthetic biology
  • Computational biology

Background:

  • Metabolic engineering aims to control microbial metabolism for producing target compounds.
  • Computational tools are crucial for identifying novel biosynthetic routes and optimizing host metabolism.

Purpose of the Study:

  • To review computational methods for exploring biologically producible compounds.
  • To outline strategies for genetic modifications and optimization criteria in metabolic engineering.
  • To discuss advancements in computational approaches for strain design.

Main Methods:

  • Exploration of native, heterologous, and broad-specificity enzymes for biosynthesis.
  • Development of computational methods for suggesting genetic modifications (deletion, addition, regulation).
  • Implementation of strategies for high yield, productivity, and substrate co-utilization.
  • Optimization of computational algorithm performance for complex strategy identification.

Main Results:

  • Computational tools can identify a wide range of compounds producible by engineered microbes.
  • Methods exist to suggest various genetic modifications for enhancing chemical production.
  • Advanced algorithms enable the discovery of more sophisticated metabolic engineering strategies.
  • Improved computational performance allows for the analysis of more complex biological systems.

Conclusions:

  • Computational approaches are powerful for designing microbial cell factories.
  • Further integration of kinetic data will enhance the predictive accuracy of metabolic engineering algorithms.
  • The field is advancing towards more efficient and targeted microbial chemical production.