Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multi-omics approach investigating thrombolysis resistance in acute ischemic stroke thrombi.

Journal of thrombosis and thrombolysis·2026
Same author

Braided Versus Laser-Cut Stents in Venous Sinus Stenting for Pulsatile Tinnitus: A Systematic Review and Meta-analysis.

AJNR. American journal of neuroradiology·2026
Same author

Evaluation of Hemodynamic Environment of Intracranial Aneurysms After Flow Disruption Based on Angiographic Signatures.

International journal for numerical methods in biomedical engineering·2026
Same author

Neutrophil extracellular trap-related pathology in sporadic brain arteriovenous malformations after radiosurgery: An exploratory histopathological study.

Clinical neurology and neurosurgery·2026
Same author

State and Diffusion of National Institutes of Health Funding of AI in Radiology.

Journal of imaging informatics in medicine·2026
Same author

Endovascular Therapy Versus Medical Therapy Alone for Basilar Artery Stroke: A Systematic Review and Meta-Analysis Through Nested Knowledge.

Stroke (Hoboken, N.J.)·2026

Related Experiment Video

Updated: Apr 18, 2026

Author Spotlight: Modeling Vascular Contributions to Alzheimer's Disease in Transgenic Mice
04:29

Author Spotlight: Modeling Vascular Contributions to Alzheimer's Disease in Transgenic Mice

Published on: May 17, 2024

1.3K

Elastase-induced intracranial dolichoectasia model in mice.

Daying Dai1, Ram Kadirvel, Issa Rezek

  • 1*Neuroradiology Research Laboratory, and ‡Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota.

Neurosurgery
|January 20, 2015
PubMed
Summary

Researchers developed a mouse model for intracranial dolichoectasia by injecting elastase. Doses of 15-25 milliunits (mu) successfully created the condition with acceptable mortality rates.

More Related Videos

Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm
08:33

Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm

Published on: August 24, 2019

8.2K
Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase
04:49

Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase

Published on: February 11, 2022

6.4K

Related Experiment Videos

Last Updated: Apr 18, 2026

Author Spotlight: Modeling Vascular Contributions to Alzheimer's Disease in Transgenic Mice
04:29

Author Spotlight: Modeling Vascular Contributions to Alzheimer's Disease in Transgenic Mice

Published on: May 17, 2024

1.3K
Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm
08:33

Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm

Published on: August 24, 2019

8.2K
Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase
04:49

Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase

Published on: February 11, 2022

6.4K

Area of Science:

  • Neurology
  • Vascular Biology
  • Animal Models

Background:

  • Intracranial dolichoectasia is a serious condition with poorly understood causes.
  • Developing animal models is crucial for studying this disease.

Purpose of the Study:

  • To establish a reliable murine model for intracranial dolichoectasia.
  • To determine optimal elastase dosage for inducing dolichoectasia in mice.

Main Methods:

  • C57/BL6 mice received varying doses of elastase (0-55 mu) via cisterna magna injection.
  • Cerebral vasculature was analyzed 14 days post-injection using MicroFil.
  • Dolichoectasia was defined by a tortuosity index >10 and arterial diameter increase >25%.

Main Results:

  • Mortality rates increased with elastase dosage, with 35 and 55 mu groups excluded.
  • Doses of 15 and 25 mu successfully induced dolichoectasia in key cerebral arteries.
  • No significant difference in efficacy was observed between 15 and 25 mu elastase groups.

Conclusions:

  • Elastase injection into the cisterna magna is an effective method for creating a murine model of intracranial dolichoectasia.
  • 15-25 mu of elastase represents a suitable dosage range, balancing efficacy with acceptable mortality.