Active opioid binding protein from rat brain--a glycoprotein containing alpha-methyl-D-mannoside residues

Active opioid binding protein was partially purified from rat brain by Vicia bungei Ohwi lectin (VBL). Mannose also existed in opioid receptors since the binding between opioid binding protein and VBL is specific, via alpha-methyl-D-mannoside (MeMan) residues. Since the active opioid binding protein purified by VBL chromatography was enriched about 200-folds. VBL can be used as effective purification tool which is much better than wheat germ agglutinin (WGA), a lectin commonly used in purifying opioid receptor.