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High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

Gene electrotransfer clinical trials.

Richard Heller¹, Loree C Heller¹

¹Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA; School of Medical Diagnostics and Translational Sciences, College of Health Sciences, Old Dominion University, Norfolk, VA, USA.

Advances in Genetics

|January 27, 2015

Summary

This summary is machine-generated.

Non-viral gene therapy uses electroporation to deliver plasmid DNA effectively. This method allows fine-tuned gene expression for therapeutic applications, showing promising results in cancer and infectious disease treatments.

Keywords:

Cancer immunotherapy Cervical cancer Clinical trials DNA vaccines Electrotransfer GET HIV Hantavirus Hepatitis B Hepatitis C Human papillomavirus In vivo electroporation Melanoma Metastatic disease Nonviral gene therapy Prostate cancer Safety and tolerability Viral vaccines

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Area of Science:

Biotechnology
Gene Therapy
Medical Devices

Background:

Non-viral gene therapy offers an alternative to viral vectors.
Electroporation, initially a lab tool, is now adapted for in vivo gene delivery.
Clinical applications have advanced from chemotherapeutics to plasmid DNA delivery.

Purpose of the Study:

To review clinical trials utilizing in vivo electroporation for plasmid DNA delivery.
To highlight the safety, tolerability, and efficacy of this gene delivery method.
To discuss the potential of electroporation in cancer therapy and vaccine development.

Main Methods:

In vivo electroporation (electrotransfer) using electric fields for plasmid DNA delivery.
Development of electrode technology for in vivo applications.
Fine-tuning of pulse protocols to control transgene expression levels and duration.

Main Results:

Over fifty clinical trials have employed electric fields for gene delivery since 2004.
Demonstrated safety and tolerability of in vivo electroporation.
Promising early clinical efficacy in cancer therapeutics and infectious disease vaccines.

Conclusions:

In vivo electroporation is a safe and effective method for plasmid DNA delivery in clinical settings.
The malleability of electroporation allows for tailored transgene expression, crucial for therapeutic success.
This technology holds significant promise for advancing cancer treatments and infectious disease vaccines.