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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Apr 18, 2026

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

Mobile flow cytometer for mHealth.

Joshua Balsam¹, Hugh Alan Bruck, Avraham Rasooly

¹Division of Biology, Office of Science and Engineering, FDA, Silver Spring, MD, 20993, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 29, 2015

Summary

This summary is machine-generated.

A new mobile flow cytometer uses a webcam for high-throughput rare cell detection, overcoming the limitations of traditional, bulky devices. This low-cost innovation enables potential mHealth applications in resource-poor settings.

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Area of Science:

Biomedical Engineering
Cell Biology
Medical Diagnostics

Background:

Traditional flow cytometry is limited by large size, high cost, and power requirements, hindering its use in mobile health (mHealth).
Current flow cytometers lack portability and have low volumetric throughput, unsuitable for rapid rare cell detection.

Purpose of the Study:

To develop a novel, low-cost, mobile flow cytometer for high-throughput fluorescence detection of rare cells.
To enable mHealth applications by creating a portable and affordable flow cytometry device.

Main Methods:

Utilized a wide-field imaging approach with a high-speed webcam (187 fps, 320x240 resolution).
Employed a 1 W, 450 nm blue laser for excitation and detected fluorescence at 535 nm.
Developed a wide-field flow cell for large volume analysis, allowing lower cell velocities.

Main Results:

The mobile flow cytometer achieved detection of low concentrations at flow rates of 500 μL/min.
Demonstrated suitability for large volume analysis and rare cell detection, simulating circulating tumor cells (CTCs).

Conclusions:

The developed mobile flow cytometer is low-cost, portable, and capable of high-throughput rare cell detection.
This device has potential clinical applications in mHealth, particularly for resource-poor settings and global health initiatives.