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Effect of acute complete obstruction on the rabbit urinary bladder
1Division of Urology, University of Pennsylvania School of Medicine, Philadelphia.
The Journal of Urology
|January 1, 1989
Summary
Complete bladder outlet obstruction in rabbits rapidly decreases bladder function and muscarinic receptor density within hours. This functional decline occurs even before significant bladder overdistension, suggesting early mechanisms are at play.
Area of Science:
- Urology
- Physiology
- Pharmacology
Background:
- Partial bladder outlet obstruction causes significant urinary bladder structural and functional changes.
- Initial bladder overdistension within 24 hours is thought to contribute to these alterations.
Purpose of the Study:
- To investigate the time course of complete bladder outlet obstruction effects on rabbit bladder contractile response and muscarinic receptor density.
- To determine if functional changes precede bladder overdistension.
Main Methods:
- Male White New Zealand rabbits underwent complete urethral obstruction.
- Groups were assessed at 4, 8, 20, and 24 hours post-obstruction.
- Contractile responses to bethanechol and field stimulation were measured.
- Muscarinic receptor density was quantified.
Main Results:
- A significant decrease in contractile response and muscarinic receptor density was observed at 4 hours, prior to overdistension.
- Both receptor density and contractile response continued to decline over the 24-hour period.
- Spontaneous contractile activity increased following obstruction.
Conclusions:
- Complete bladder outlet obstruction rapidly impairs bladder contractile function and reduces muscarinic receptor density.
- Early functional deficits occur independently of bladder overdistension.
- High spontaneous contractile activity may contribute to the initial decrease in receptor density and function.