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Related Concept Videos

Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Updated: Apr 18, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Proteomics beyond large-scale protein expression analysis.

Paul J Boersema1, Abdullah Kahraman2, Paola Picotti1

  • 1Institute of Biochemistry, Department of Biology, ETH Zurich, Otto-Stern-Weg 3, CH-8093 Zurich, Switzerland.

Current Opinion in Biotechnology
|January 31, 2015
PubMed
Summary
This summary is machine-generated.

Proteomics has evolved beyond simple protein expression analysis. New workflows now enable detailed studies of protein dynamics, structure, activity, and interactions for systems biology.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Systems Biology

Background:

  • Proteomics traditionally focuses on protein expression analysis.
  • Common outputs include protein inventories and differential expression lists.

Purpose of the Study:

  • To highlight the expansion of proteomics beyond expression analysis.
  • To introduce novel proteomics workflows and their applications.

Main Methods:

  • Targeted proteomics for dynamic pathway and network analysis.
  • Structural proteomics for structural modeling and identification.
  • Other workflows for protein activity, localization, degradation, and turnover analysis.

Main Results:

  • Expanded capabilities in analyzing protein systems dynamics.
  • Enabled large-scale assessment of protein activity, location, and turnover.
  • Provided tools for multi-level 'omics' integration.

Conclusions:

  • Proteomics now offers advanced tools for comprehensive biological network modeling.
  • These developments are crucial for advancing systems biology research.
  • The field has significantly broadened its scope and analytical power.