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Transduction

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...

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Updated: Apr 17, 2026

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Published on: March 10, 2017

Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method.

Kelsey L Krausz¹, Jeffrey L Bose²

¹Department of Microbiology, Molecular Genetics, and Immunology, The University of Kansas Medical Center, MSN 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 4, 2015

Summary

This summary is machine-generated.

This study presents a simple broth-based method for transferring DNA between Staphylococcus aureus strains. This transduction technique facilitates genetic manipulation in Staphylococci, moving plasmids and chromosomal DNA effectively.

Keywords:

Bacteriophage Transduction phi11 phi80α

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Area of Science:

Microbiology
Molecular Biology
Bacteriology

Background:

Genetic manipulation of Staphylococcus strains is crucial for research.
Transduction using generalized transducing phage is a common method for DNA transfer in Staphylococci.

Purpose of the Study:

To describe an accessible, broth-based transduction protocol for Staphylococcus aureus.
To enable efficient transfer of plasmids and chromosomal DNA between strains.

Main Methods:

A broth-based transduction procedure was employed.
The method was validated for moving both plasmids and chromosomal fragments.

Main Results:

The protocol is relatively easy to perform.
The method has been used extensively for DNA transfer in Staphylococcus aureus.

Conclusions:

The described transduction method is effective for genetic manipulation of Staphylococcus aureus.
This protocol offers a practical approach for transferring genetic material between strains.