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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

MicroRNAs

MicroRNAs

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Related Experiment Video

Updated: Apr 17, 2026

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Published on: June 16, 2023

MicroRNAs in osteosarcoma.

Jian Zhang¹, Yi-Guo Yan¹, Cheng Wang¹

¹Department of Spine Surgery, The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China.

Clinica Chimica Acta; International Journal of Clinical Chemistry

|February 10, 2015

Summary

Osteosarcoma (OS) treatment needs new approaches due to chemotherapy resistance. MicroRNAs (miRNAs) show promise as therapeutic targets and biomarkers for this bone cancer.

Keywords:

Chemoresistance Notch OS TP53 miRNAs

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Last Updated: Apr 17, 2026

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

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Published on: June 16, 2023

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

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MeRIP-qPCR Assay for Detecting m6A Modification Levels of Specific RNA in Osteosarcoma Cells

MeRIP-qPCR Assay for Detecting m⁶A Modification Levels of Specific RNA in Osteosarcoma Cells

Published on: December 30, 2025

Area of Science:

Oncology
Molecular Biology
Biochemistry

Background:

Osteosarcoma (OS) is a primary bone cancer primarily affecting children and adolescents, with poor prognosis linked to chemotherapy resistance.
MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression, implicated in various cancers, including OS.
Differential expression of miRNAs in OS tissues and serum suggests their role as oncogenes or tumor suppressors.

Purpose of the Study:

To review miRNA biochemistry, focusing on their expression profiles, roles in osteosarcoma development, and therapeutic potential.
To highlight the significance of understanding miRNA alterations for identifying novel biomarkers.
To explore the development of miRNA-targeted therapies for osteosarcoma.

Main Methods:

Literature review of miRNA biochemistry and function in osteosarcoma.
Analysis of miRNA expression profiles in OS tissues, cell lines, and serum.
Evaluation of the therapeutic potential of miRNA restoration in OS models.

Main Results:

miRNAs are differentially expressed in OS and influence tumorigenesis.
Altered miRNAs can act as oncogenes or tumor suppressors in osteosarcoma.
Restoring miRNA expression demonstrates therapeutic promise for OS treatment.

Conclusions:

Understanding miRNA expression and function is crucial for osteosarcoma research.
miRNAs hold potential as diagnostic biomarkers and therapeutic targets for osteosarcoma.
miRNA-targeted therapies represent a promising avenue for improving OS patient outcomes.