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Applicability evaluation of Deep Eutectic Solvents-Cellulase system for lignocellulose hydrolysis
Ahmad Anas Nagoor Gunny1, Dachyar Arbain1, Enas Muen Nashef2
1School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
Bioresource Technology
|February 10, 2015
Summary
Deep Eutectic Solvents (DESs) show promise for lignocellulose pretreatment, maintaining high cellulase activity and improving glucose production. Ethylene glycol-based DES (EG) demonstrated superior performance over glycerol-based DES (GLY) and alkali systems.
Area of Science:
- Biotechnology
- Biomass Conversion
- Green Chemistry
Background:
- Deep Eutectic Solvents (DESs) are emerging as advanced ionic liquids for lignocellulose pretreatment.
- A key challenge is the potential inactivation of cellulase enzymes by DES salt components during saccharification.
Purpose of the Study:
- To evaluate the stability and applicability of DESs in conjunction with cellulase for lignocellulose saccharification.
- To compare the performance of DES-based systems against traditional diluted alkali pretreatment.
Main Methods:
- Assessing cellulase stability in the presence of selected DESs (GLY and EG).
- Evaluating glucose production, energy consumption, and kinetic performance of DES-Cellulase systems.
- Comparing DES systems with a diluted alkali pretreatment system.
Main Results:
- Cellulase retained over 90% activity with 10% (v/v) GLY and EG DES.
- Both DES systems yielded higher glucose enhancement and lower energy consumption than diluted alkali.
- EG-based DES exhibited superior kinetic performance compared to GLY-based DES.
Conclusions:
- DESs are suitable for lignocellulose pretreatment, showing good cellulase compatibility and enhanced saccharification efficiency.
- EG-based DES offers a promising alternative to conventional pretreatment methods due to its effectiveness and energy efficiency.
