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Updated: Apr 17, 2026

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
Published on: October 24, 2014
High-frequency multimodal atomic force microscopy
Adrian P Nievergelt1, Jonathan D Adams1, Pascal D Odermatt1
1Laboratory for Bio- and Nano-Instrumentation, École Polytechnique Fédérale de Lausanne, Batiment BM 3109 Station 17, 1015 Lausanne, Switzerland.
Abstract:
Multifrequency atomic force microscopy imaging has been recently demonstrated as a powerful technique for quickly obtaining information about the mechanical properties of a sample. Combining this development with recent gains in imaging speed through small cantilevers holds the promise of a convenient, high-speed method for obtaining nanoscale topography as well as mechanical properties. Nevertheless, instrument bandwidth limitations on cantilever excitation and readout have restricted the ability of multifrequency techniques to fully benefit from small cantilevers. We present an approach for cantilever excitation and deflection readout with a bandwidth of 20 MHz, enabling multifrequency techniques extended beyond 2 MHz for obtaining materials contrast in liquid and air, as well as soft imaging of delicate biological samples.

