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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
Published on: June 16, 2017
DNA transfection into mammalian cells, selective markers and gene cointegration
F Colbère-Garapin1, M L Ryhiner, A C Garapin
1Unité de Virologie Médicale, Institut Pasteur, Paris, France.
Developments in Biological Standardization
|January 1, 1989
Abstract:
The dominant selective markers that we and others have constructed for the selection of cells which stably acquired a foreign DNA, are briefly described. The possibility of obtaining the cointegration of several genes, and of inducing their amplification in the recipient cell, is discussed.
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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.