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Updated: Apr 17, 2026

Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst
Byeonghun Lee1, Saebom Lee, Hyeonsoo Kim
1Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea.
Objective:
To produce 10-ketostearic acid from oleic acid.
Results:
Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 °C with 5 g cells l(-1) and 2.5 g oleic acid l(-1). Under these conditions, the cells produced 1.96 g 10-ketostearic acid l(-1) from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l(-1) h(-1).
Conclusion:
This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum.
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