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Influenza Virus Propagation in Embryonated Chicken Eggs
Published on: March 19, 2015
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Isolation and propagation of coronaviruses in embryonated eggs
1Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27606, USA, jsguy@ncsu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2015
Summary
Embryonated eggs support the replication of avian coronaviruses like infectious bronchitis virus (IBV) and turkey coronavirus (TCoV). Different inoculation routes are optimal depending on the specific coronavirus, highlighting the egg
Area of Science:
- Virology
- Embryology
Background:
- Embryonated eggs offer diverse cell types crucial for viral replication.
- Avian coronaviruses, particularly within the Gammacoronavirus genus, utilize embryonated eggs for propagation.
Purpose of the Study:
- To investigate the suitability of embryonated eggs as a host system for avian coronaviruses.
- To determine optimal inoculation routes for different Gammacoronavirus species.
Main Methods:
- Inoculation of embryonated chicken eggs with infectious bronchitis virus (IBV), turkey coronavirus (TCoV), and pheasant coronavirus.
- Assessment of viral replication and titers based on inoculation route (allantoic vs. amniotic).
Main Results:
- IBV and pheasant coronavirus efficiently replicate in embryonated chicken eggs, with the allantoic route yielding high titers.
- Turkey coronavirus (TCoV) replication is restricted to the embryo's intestinal epithelium, necessitating amniotic inoculation.
Conclusions:
- Embryonated eggs are a versatile host system for avian Gammacoronavirus isolation and propagation.
- Specific inoculation routes are critical for efficient propagation of different avian coronaviruses.
- Embryonated eggs hold potential for detecting novel coronaviruses.
