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[Morphological properties and adhesiveness of bacteria of the genus Proteus]
Abstract:
Out of 100 Proteus strains isolated from patients with purulent inflammatory, urological and enteric diseases, from healthy persons and from the environment, 29 stains showed the positive D-mannose-resistant reaction of hemagglutination with chick red blood cells and 18 strains showed such reaction with goose and duck red blood cells. The results of these studies permit the use of chick red blood cells as target cells for the detection of Proteus adhesin. Human red blood cells of groups O, A, B and AB, sheep, bovine, dog, rat and rabbit red blood cells gave no positive D-mannose-sensitive reaction and D-mannose-resistant reaction of hemagglutination. In bacterial cells pili function as organelles which determine Proteus adhesiveness, while flagellae play no positive role.
Insights
Proteus strains exhibit D-mannose-resistant hemagglutination with avian red blood cells, suggesting chick red blood cells are suitable for detecting Proteus adhesin. Bacterial pili, not flagellae, facilitate this adhesiveness.
Area of Science:
- Microbiology
- Bacterial Adhesion Mechanisms
Background:
- Proteus strains are associated with various human diseases and environmental presence.
- Bacterial adhesins play a crucial role in colonization and pathogenesis.
- Understanding the specific adhesins of Proteus is important for diagnostics and treatment.
Purpose of the Study:
- To investigate the hemagglutination properties of Proteus strains.
- To identify suitable target cells for detecting Proteus adhesin.
- To elucidate the role of bacterial appendages in Proteus adhesiveness.
Main Methods:
- Isolation and characterization of 100 Proteus strains from diverse sources.
- Hemagglutination assays using various red blood cells (chick, goose, duck, human, sheep, bovine, dog, rat, rabbit).
- Testing for both D-mannose-sensitive and D-mannose-resistant reactions.
Main Results:
- 29 out of 100 Proteus strains showed D-mannose-resistant hemagglutination with chick red blood cells.
- 18 strains exhibited D-mannose-resistant hemagglutination with goose and duck red blood cells.
- No positive reactions were observed with human or other mammalian red blood cells.
- Bacterial pili were identified as the organelles responsible for Proteus adhesiveness, while flagellae did not play a significant role.
Conclusions:
- Chick red blood cells are effective target cells for detecting Proteus adhesin.
- The D-mannose-resistant hemagglutination is specific to avian red blood cells.
- Proteus adhesiveness is mediated by pili, highlighting their importance in bacterial interactions.