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Clinical and microbiological characteristics of six Staphylococcus pettenkoferi isolates from blood samples

Sholhui Park1, Hae-Sun Chung1, Miae Lee1

  • 1Department of Laboratory Medicine, Ewha Womans University School of Medicine, Seoul, Korea.

Annals of Laboratory Medicine
|March 3, 2015
PubMed

Insights

Staphylococcus pettenkoferi, a novel coagulase-negative staphylococci (CoNS) species, is increasingly identified in bloodstream infections. Accurate identification requires advanced methods beyond conventional phenotypic tests.

Area of Science:

  • Clinical microbiology
  • Infectious diseases
  • Bacterial identification

Background:

  • Coagulase-negative staphylococci (CoNS) are a significant cause of hospital-acquired bloodstream infections.
  • Staphylococcus pettenkoferi, identified in 2002, is a less-characterized CoNS species.
  • Accurate identification of novel bacterial species is crucial for effective patient management.

Observation:

  • Six Staphylococcus pettenkoferi strains were isolated from blood specimens.
  • One isolate was pathogenic, while five were contaminants or catheter colonizers.
  • Conventional phenotypic identification methods proved unreliable for these isolates.

Findings:

  • Brucker Biotyper and 16S rRNA gene sequencing confirmed all six isolates as Staphylococcus pettenkoferi.
  • Five of the six isolates exhibited resistance to oxacillin.
  • All isolates remained susceptible to vancomycin and linezolid.

Implications:

  • Advanced identification techniques, such as MALDI-TOF MS (Brucker Biotyper) and 16S rRNA sequencing, are essential for accurate Staphylococcus pettenkoferi detection.
  • Understanding the antimicrobial resistance patterns of S. pettenkoferi is vital for guiding treatment strategies.
  • Improved diagnostic methods are needed to differentiate true pathogens from colonizers in clinical settings.
Keywords:
16S ribosomal RNAMALDI-TOF MSStaphylococcus pettenkoferi

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