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Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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Related Experiment Video

Updated: May 13, 2026

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

Published on: February 10, 2011

Practical Considerations for Reporting Staphylococcus epidermidis Using BioFire FilmArray Blood Culture

Seunghwan Kim¹, Hae-Sun Chung¹, Min-Kyung So¹

¹Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, Korea.

Annals of Laboratory Medicine

|May 11, 2026

Summary

This summary is machine-generated.

Analyzing the difference in crossing point (ΔCp) values with multicolony subculture can improve the interpretation of BioFire FilmArray Blood Culture Identification 2 (BCID2) results for Staphylococcus epidermidis in polymicrobial blood cultures.

Keywords:

Blood culture identification Coagulase-negative staphylococci Multiplex PCR

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Last Updated: May 13, 2026

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Published on: May 24, 2024

Area of Science:

Clinical microbiology
Molecular diagnostics
Infectious disease diagnostics

Background:

The BioFire FilmArray Blood Culture Identification 2 (BCID2) panel rapidly detects bloodstream pathogens.
Discrepancies can occur between BCID2 results and standard cultures for coagulase-negative staphylococci (CoNS), particularly Staphylococcus epidermidis.

Purpose of the Study:

To evaluate the utility of analyzing ΔCp values and multicolony subculture for improving BCID2 result interpretation in cases of suspected S. epidermidis.
To assess the accuracy of BCID2 in identifying S. epidermidis in polymicrobial blood cultures.

Main Methods:

Retrospective analysis of BCID2 results from 20 patients with S. epidermidis detection.
Comparison of BCID2 findings with standard blood culture and multicolony subculture identification.
Analysis of ΔCp values (Staphylococcus genus vs. S. epidermidis target) in BIOFIRE FIREWORKS software.

Main Results:

Conventional blood culture confirmed S. epidermidis in 12 cases; eight showed other CoNS.
Multicolony subculture revealed S. epidermidis co-detection in six of the discrepant cases.
Significantly higher ΔCp values were observed in discrepant cases compared to concordant cases (median 3.55 vs. -0.15 cycles; P=0.0002).

Conclusions:

ΔCp analysis, when combined with multicolony subculture, can aid in identifying potential BCID2-culture discrepancies in mixed CoNS infections.
This approach may enhance the accurate identification of S. epidermidis in complex blood culture scenarios.
Improved interpretation of molecular diagnostic results is crucial for effective patient management.