Multiphoton microscopy: applications in Urology and Andrology

Area of Science:

• Urology
• Medical Imaging
• Cellular Biology

Background:

• Multiphoton microscopy (MPM) provides submicron resolution for real-time cellular imaging.
• Current applications include neuroscience, oncology, and immunology.
• MPM shows significant potential in various urological applications.

Purpose of the Study:

• To explore the utility of MPM in urological diagnostics and surgical guidance.
• To highlight MPM's capabilities in identifying key structures and pathologies in urological tissues.

Main Methods:

• Utilized MPM for in vivo visualization of cellular processes.
• Applied MPM in preclinical models for testicular, bladder, and prostate tissue analysis.

Main Results:

• Successfully visualized seminiferous tubules and spermatogenesis in a rat model.
• MPM effectively identified suspicious malignancy in bladder cancer evaluations.
• Demonstrated utility in differentiating prostate tissue from critical nerves during simulated prostatectomy.

Conclusions:

• MPM is a promising imaging tool for urological applications, including fertility assessment and cancer diagnosis.
• Potential for real-time intraoperative guidance in bladder and prostate cancer surgery.
• Further safety studies are required for clinical translation of MPM in urology.