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Mutations at calcium binding site III in cyclodextrin glycosyltransferase improve β-cyclodextrin specificity

Xiaofeng Ban1, Zhengbiao Gu2, Caiming Li1

  • 1School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

International Journal of Biological Macromolecules
|March 10, 2015
PubMed
Summary

Modifying a specific site on cyclodextrin glycosyltransferases (CGTases) with charged amino acids significantly enhances beta-cyclodextrin production. These engineered CGTases show improved specificity and are more suitable for industrial applications.

Keywords:
Calcium binding siteCyclodextrin glycosyltransferaseProduct specificityResidue 315β-Cyclodextrin

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