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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

3.6K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
3.0K
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

10.1K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
10.1K
Types Of Column Chromatography01:29

Types Of Column Chromatography

15.3K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
15.3K
Chromatography: Introduction01:10

Chromatography: Introduction

8.6K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

4.4K
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
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Affinity chromatography: a historical perspective.

David S Hage1, Ryan Matsuda

  • 1Department of Chemistry, University of Nebraska-Lincoln, 704 Hamilton Hall, 639 N 12 Street, Lincoln, NE, 68588-0304, USA, dhage1@unl.edu.

Methods in Molecular Biology (Clifton, N.J.)
|March 10, 2015
PubMed
Summary
This summary is machine-generated.

Affinity chromatography uses selective binding agents for separating complex mixtures. This review covers its principles, developments, and diverse applications in chemical analysis.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Separation Science

Background:

  • Affinity chromatography is a highly selective liquid chromatography technique.
  • It utilizes a biologically related stationary phase for specific target molecule binding.
  • This method is crucial for separating and analyzing components in complex mixtures.

Observation:

  • The review traces the historical evolution of affinity chromatography.
  • It details advancements in binding agents, support materials, and immobilization techniques.
  • Current methodologies and their underlying principles are discussed.

Findings:

  • Significant progress has been made in developing novel affinity ligands and supports.
  • Immobilization strategies have been refined for enhanced column performance.
  • The technique's versatility is demonstrated through various successful applications.

Implications:

  • Affinity chromatography has revolutionized chemical separation and analysis.
  • It has paved the way for the development of other affinity-based methods.
  • Its continued evolution promises further advancements in molecular separation technologies.