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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Overview Of Cell Separation And Isolation01:20

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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Cell Capture Using a Microfluidic Device
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Cell Capture Using a Microfluidic Device

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Cell affinity separations on microfluidic devices.

Yan Gao1, Wenjie Li, Ye Zhang

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Office Chemistry 300-B, Lubbock, TX, 79409-1061, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 10, 2015
PubMed
Summary
This summary is machine-generated.

Microfluidic affinity cell chromatography offers a label-free method for separating cells from complex samples. This chapter details protocols for cell separation using polydimethylsiloxane (PDMS)-glass microdevices and glass capillaries.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Cell separation is crucial for biological and medical research.
  • Microfluidic devices offer advantages for cell analysis.
  • Label-free separation techniques are highly desirable.

Purpose of the Study:

  • To describe protocols for microfluidic affinity cell separation.
  • To detail cell separation in polydimethylsiloxane (PDMS)-glass microdevices.
  • To outline cell separation in glass capillaries.

Main Methods:

  • Microfluidic affinity cell chromatography.
  • Utilizing polydimethylsiloxane (PDMS)-glass microdevices.
  • Employing glass capillaries for cell separation.

Main Results:

  • Demonstration of label-free cell separation.
  • Ease of operation and rapid analysis achieved.
  • Low-cost methodology presented.

Conclusions:

  • Microfluidic affinity cell chromatography is an effective separation technique.
  • Protocols are provided for PDMS-glass microdevices and glass capillaries.
  • This method facilitates biological and medical research.