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Related Experiment Videos

Activin B: precursor sequences, genomic structure and in vitro activities.

A J Mason1, L M Berkemeier, C H Schmelzer

  • 1Department of Developmental Biology, Recovery Process R & D Sciences, Genentech Inc., South San Francisco, California 94080.

Molecular Endocrinology (Baltimore, Md.)
|September 1, 1989
PubMed
Summary
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Researchers determined the human inhibin beta B-subunit sequence, revealing its structure and function. This inhibin subunit forms activin B, which stimulates follicle-stimulating hormone (FSH) release and hemoglobin production.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Protein Chemistry

Background:

  • Inhibins are dimeric glycoproteins involved in regulating reproductive functions.
  • The beta B-subunit is a key component in the formation of activins, which are related to the transforming growth factor-beta superfamily.

Purpose of the Study:

  • To determine the complete amino acid sequence of the human inhibin beta B-subunit.
  • To investigate the biological activity of the activin B homodimer.

Main Methods:

  • Deduction of amino acid sequence from cDNA and genomic clones.
  • Expression of beta B-subunit in mammalian tissue culture cells.
  • In vitro pituitary assay for FSH release and K562 cell assay for hemoglobin accumulation.

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Main Results:

  • The complete amino acid sequence of the human inhibin beta B-subunit was elucidated.
  • Expression of the beta B-subunit resulted in the secretion of activin B homodimers.
  • Activin B demonstrated potent FSH-releasing and hemoglobin accumulation activity, comparable to activin A.

Conclusions:

  • The human inhibin beta B-subunit sequence provides insights into its structure and processing.
  • Activin B is a biologically active molecule with functions in the pituitary and erythroid differentiation.
  • Activin B plays a significant role in regulating physiological processes, similar to activin A.