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A user's guide to the Arabidopsis T-DNA insertion mutant collections
Ronan C O'Malley1, Cesar C Barragan, Joseph R Ecker
1Genomic Analysis Laboratory, Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA, 92037, USA, omalley@salk.edu.
Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2015
Summary
This chapter guides researchers on effectively using Arabidopsis thaliana T-DNA mutant collections for gene function studies. It details selecting mutant lines and implementing T-DNA genotyping pipelines for plant biology research.
Area of Science:
- Plant Biology
- Genetics
- Molecular Biology
Background:
- Arabidopsis thaliana T-DNA sequence-indexed mutant collections are crucial resources for plant gene function research.
- These collections provide insertional mutants for most genes, enabling in vivo characterization.
Purpose of the Study:
- To provide a comprehensive guide for effectively utilizing T-DNA mutant collections.
- To assist researchers in selecting appropriate mutant lines and designing genotyping pipelines.
Main Methods:
- Introduction to T-DNA insertional mutant collections and data sources.
- Step-by-step guide for designing and implementing T-DNA genotyping pipelines.
- Methods for interpreting genotyping results and troubleshooting segregation distortions.
Main Results:
- Effective strategies for selecting suitable mutant lines are presented.
- A practical framework for establishing T-DNA genotyping pipelines is provided.
- Guidance on interpreting results and addressing common genotyping challenges is offered.
Conclusions:
- The chapter empowers both new and experienced users to leverage T-DNA mutant collections efficiently.
- Successful implementation of genotyping pipelines facilitates robust gene function studies in Arabidopsis.
- This resource enhances the utility of mutant collections for diverse plant biology research questions.
