Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

22.2K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
22.2K
DNA Microarrays02:34

DNA Microarrays

23.3K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
23.3K
Sanger Sequencing01:57

Sanger Sequencing

780.8K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
780.8K
Next-generation Sequencing03:00

Next-generation Sequencing

102.1K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
102.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic technologies to enhance crop nutritional value under climate change.

Nature·2026
Same author

Bioluminescent sentinel plants enable autonomous diagnostics of viral infections.

Nature communications·2026
Same author

Virus induced gene editing using potyviral vectors in Cas12a expressing plants.

Horticulture research·2026
Same author

Editing strigolactone biosynthesis genes in tomato reveals novel phenotypic effects and highlights D27 as a breeding target for parasitic weed resistance.

Plant & cell physiology·2026
Same author

Optimizing a tomato crocin biofactory by fine-tuning plant architecture.

Frontiers in plant science·2026
Same author

Knockout of a pectate lyase in tomato increases fruit firmness and reduces foliar susceptibility to pathogens.

Plant science : an international journal of experimental plant biology·2025
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

15.4K

Software-assisted stacking of gene modules using GoldenBraid 2.0 DNA-assembly framework.

Marta Vazquez-Vilar1, Alejandro Sarrion-Perdigones, Peio Ziarsolo

  • 1Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2015
PubMed
Summary
This summary is machine-generated.

GoldenBraid (GB) is a modular DNA assembly technology that accelerates plant multigene engineering. This software-assisted method enables rapid stacking of genes for applications like polyphenol metabolic engineering.

More Related Videos

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

17.5K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.3K

Related Experiment Videos

Last Updated: Apr 16, 2026

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

15.4K
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

17.5K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.3K

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Plant Biotechnology

Background:

  • Efficient assembly of multiple genes into a single construct is crucial for plant metabolic engineering.
  • Existing methods can be time-consuming and complex, limiting the rapid development of engineered plant systems.

Purpose of the Study:

  • To describe the GoldenBraid (GB) modular DNA assembly technology for plant multigene engineering.
  • To detail the methodology for creating a three-transcriptional unit multigene construct using GB for polyphenol metabolic engineering.

Main Methods:

  • Utilizing type IIS restriction enzymes for modular DNA assembly.
  • Employing a suite of software tools (GBDomesticator, GB TU Assembler, GB Binary Assembler) for DNA part adaptation and construct assembly.
  • Demonstrating the method by assembling a three-gene construct for plant polyphenol biosynthesis.

Main Results:

  • GoldenBraid (GB) technology significantly speeds up the assembly of transcriptional units and gene stacking within T-DNA constructs.
  • The software-assisted tools streamline the process, enabling the creation of complex multigene constructs in days.
  • Successful assembly of a three-transcriptional unit construct for polyphenol metabolic engineering was achieved.

Conclusions:

  • GoldenBraid (GB) provides an efficient, modular, and software-assisted approach for plant multigene engineering.
  • This technology facilitates rapid development of plant metabolic engineering applications, such as polyphenol production.
  • The GB system, with its online tools, democratizes complex DNA assembly for plant science research.