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Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Published on: November 9, 2014
Fluorescence intensity of composite layering combined with surface sealant submitted to staining solutions
Anderson Catelan1, Ana Paula Albuquerque Guedes, Thaís Yumi Umeda Suzuki
1Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
Purpose:
The aim of this study was to evaluate the fluorescence intensity of a composite in different layering techniques combined to a surface sealant submitted to staining solutions.
Methods And Materials:
One surface sealant and one composite resin (shades A2-0, A2, and YE) were tested. Specimens were carried out using layering with different shades of composite, and dental blocks (dentin/enamel) were obtained of human molars. Fluorescence intensity measurement performed using a spectrofluorophotometer, before and after storage in cola, orange juice, red wine, and distilled water. Data were subjected to analysis of variance and Fisher's test (α = 0.05).
Results:
Initially, only A2-O + A2 layering showed fluorescence intensity statistically similar to the natural tooth. Overall, groups sealed showed the highest fluorescence intensity compared with unsealed groups. The aging decreased the fluorescence intensity compared with initial values. Red wine showed the highest decrease on the fluorescence intensity.
Conclusion:
The fluorescence of composite restorations is dependent of the layering technique and is affected by the diet.
Clinical Significance:
The staining solutions decreased the fluorescence intensity, even when the composite resins were sealed. Different shades of the same composite showed distinct fluorescence values, so there should be greater standardization of this property for tooth reconstruction success by the layering technique with large tissue loss.
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