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Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
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An aryl-thioether substituted nitrobenzothiadiazole probe for the selective detection of cysteine and homocysteine
Dayoung Lee1, Gyoungmi Kim, Jun Yin
1Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120-750, Korea. jyoon@ewha.ac.kr.
Abstract:
An aryl-thioether substituted nitrobenzothiadiazole probe was synthesized and employed to detect cysteine and homocysteine selectively in living cells. Interestingly, both cysteine (Cys) and homocysteine (Hcy) promote an enhancement of the fluorescence intensity of the probe at pH 7.4 while only Cys gives rise to this enhancement under weakly acidic conditions (pH 6.0).

