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Updated: Apr 16, 2026
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
Published on: March 21, 2018
Three-wavelength light control of freely moving Drosophila Melanogaster for less perturbation and efficient
Yen-Yin Lin1, Ming-Chin Wu2, Po-Yen Hsiao3
1Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
Abstract:
We developed a real-time automated laser-tracking system combined with continuous wave 1064-nm infrared or 473-nm blue lasers to provide punishment for studying memory in Drosophila Melanogaster. Combining optogenetic tools with laser properties, such as 473-nm and 593-nm lasers that activate light sensitive proteins in artificial transgenic flies, we can manipulate the specific neuron of an assigned fly among multiple flies to investigate neuron circuit relationships in social interactions. In restraining condition assay or optogenetic experiments, a ventral irradiated system would be more efficient due to higher ventral cuticle transmissions and neuron ganglia locations. Therefore, ventral irradiated systems cause less perturbation during behavior studies.
