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Portable microfluidic integrated plasmonic platform for pathogen detection.

Onur Tokel1, Umit Hakan Yildiz2, Fatih Inci2

  • 1Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Scientific Reports
|March 25, 2015
PubMed
Summary

A new portable microfluidic-integrated surface plasmon resonance (SPR) platform offers rapid detection of bacteria like Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) at the point-of-care (POC). This inexpensive technology addresses the need for faster infectious disease diagnosis in resource-limited settings.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Biosensing

Background:

  • Timely detection of infectious agents is crucial for effective disease diagnosis and treatment.
  • Conventional methods (ELISA, culturing, PCR) are time-consuming, expensive, and unsuitable for point-of-care (POC) applications.
  • A need exists for rapid, simple, and accurate pathogen detection methods for POC settings.

Purpose of the Study:

  • To develop a portable, multiplex, and inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform.
  • To enable rapid detection and quantification of bacterial pathogens at the POC.

Main Methods:

  • Development of a microfluidic-integrated SPR platform.
  • Testing the platform's ability to capture and detect Escherichia coli (E. coli) in phosphate-buffered saline (PBS) and peritoneal dialysis (PD) fluid.
  • Evaluating the platform's multiplexing and specificity for Staphylococcus aureus (S. aureus).

Main Results:

  • The platform demonstrated reliable capture and detection of E. coli across a concentration range of approximately 10^5 to 3.2 × 10^7 Colony Forming Units (CFUs)/mL.
  • Successful testing of multiplexing and specificity capabilities with S. aureus samples.
  • The platform achieved rapid detection of target bacteria.

Conclusions:

  • The developed SPR platform is a promising technology for rapid, multiplexed bacterial detection at the POC.
  • This technology can be adapted for detecting other pathogens in primary care and resource-constrained settings.
  • It addresses the unmet need for accessible and efficient infectious disease diagnostics.