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Both ERK1 and ERK2 are required for enterovirus 71 (EV71) efficient replication

Meng Zhu1, Hao Duan2, Meng Gao3

  • 1Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100191, China. mengzhu1984@gmail.com.

Viruses
|March 25, 2015
PubMed

Insights

Both ERK1 and ERK2 are crucial for enterovirus 71 (EV71) replication, with the late phase of their activation being particularly important for the virus life cycle.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Signaling

Background:

  • The Raf-MEK-ERK1/2 pathway is vital for viral propagation.
  • MEK1 is essential for EV71 replication, but the roles of ERK1 and ERK2 remain unclear.

Purpose of the Study:

  • To investigate the distinct functions of ERK1 and ERK2 in EV71 replication.
  • To determine the importance of ERK1/2 activation phases in the EV71 life cycle.

Main Methods:

  • Used specific ERK small interfering RNAs (siRNAs) for gene silencing.
  • Applied the selective MEK inhibitor U0126.
  • Assessed EV71 propagation, RNA amplification, and protein synthesis.

Main Results:

  • Silencing ERK1 or ERK2 significantly impaired EV71 propagation, RNA amplification, and protein synthesis.
  • ERK1 and ERK2 activations were independent and non-discriminative during EV71 infection.
  • Inhibiting the late phase of ERK1/2 activation was sufficient to suppress EV71 replication.

Conclusions:

  • Both ERK1 and ERK2 are essential and non-interchangeable for efficient EV71 replication.
  • The late phase of ERK1/2 activation plays a critical role in the EV71 life cycle.
  • Confirms the significance of the MEK1-ERK1/2 pathway in EV71 replication.

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