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Related Concept Videos

Autophagy01:27

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Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
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Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
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Related Experiment Video

Updated: Apr 15, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
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Measuring autophagy in stressed cells.

Marina N Sharifi1, Erin E Mowers, Lauren E Drake

  • 1The Ben May Department for Cancer Research, The Gordon Center for Integrative Sciences, The University of Chicago, 929 East 57th Street, Chicago, IL, 60637, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 26, 2015
PubMed
Summary
This summary is machine-generated.

Measuring cellular autophagy flux is crucial for understanding cell health and disease. This study details methods to quantify autophagy, including LC3 processing and other markers, and discusses in vivo techniques.

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Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Macro-autophagy is a vital cellular catabolic process.
  • It degrades toxic aggregates and recycles cellular components, crucial for cell survival under stress.
  • Understanding autophagic flux is key to comprehending cellular function and disease.

Purpose of the Study:

  • To describe key methodologies for measuring autophagic flux.
  • To outline methods for inducing autophagy and controlling for flux changes.
  • To detail techniques for monitoring autophagy in vivo.

Main Methods:

  • Western blot analysis of LC3 processing.
  • Fluorescent cell staining and flow cytometry.
  • Monitoring autophagy markers like p62/Sqstm1 and Atg5-Atg12 conjugate.

Main Results:

  • Established protocols for measuring autophagic flux using various techniques.
  • Identified cellular stresses that induce autophagy.
  • Provided methods to differentiate flux changes from flux inhibition.

Conclusions:

  • Accurate measurement of autophagic flux is essential for research.
  • A range of techniques are available for in vitro and in vivo autophagy assessment.
  • This work provides a comprehensive guide for researchers studying autophagy.