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Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
Published on: October 13, 2017
A bead-based multiplex sandwich immunoassay to assess the abundance and posttranslational modification state of
Nicola Groll1, Cornelia Sommersdorf, Thomas O Joos
1Department of Protein Analytics, NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr, 55, 72770, Reutlingen, Germany.
Abstract:
A system-wide analysis of cell signaling involves detecting and quantifying a range of proteins and their posttranslational modification states in the same cellular sample. We propose a protocol for a miniaturized, bead-based array and describe its efficiency in characterizing the different forms and functions of β-catenin. The protocol provides detailed instructions for cell culture and bead array assays that enable insights into complex networks at the systems level.
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