Plasma jet's shielding gas impact on bacterial inactivation
Helena Jablonowski1, Mareike A Ch Hänsch2, Mario Dünnbier1
1Center for Innovation Competence plasmatis, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany and Leibniz Institute for Plasma Science and Technology, INP Greifswald e.V., Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
Biointerphases
|April 3, 2015
Summary
Plasma medicine can selectively kill bacteria like E. coli while sparing human cells. Controlling plasma composition, specifically reactive nitrogen and oxygen species, is key to achieving targeted inactivation for wound healing applications.
Area of Science:
- Plasma medicine
- Microbiology
- Biophysics
Background:
- Plasma medicine aims to selectively eliminate prokaryotic cells while preserving or enhancing eukaryotic cell function.
- Controlling plasma composition is crucial for achieving desired therapeutic effects in plasma medicine.
Purpose of the Study:
- To investigate the inactivation of Escherichia coli (E. coli) using plasma jet treatment.
- To determine the correlation between plasma reactive species composition and bacterial inactivation efficacy.
- To identify plasma conditions that maximize bacterial inactivation while minimizing harm to eukaryotic cells.
Main Methods:
- Utilized a plasma jet device with a shielding gas to control reactive species composition in liquids.
- Generated either reactive nitrogen species (RNS)-dominated or reactive oxygen species (ROS)-dominated plasma conditions.
- Quantified bacterial inactivation rates for E. coli under different plasma conditions.
Main Results:
- Observed a strong correlation between the composition of reactive species and the inactivation of E. coli.
- Demonstrated that specific plasma compositions can achieve potent bacterial inactivation.
- Showed that it is possible to minimize adverse effects on eukaryotic cells simultaneously.
Conclusions:
- Plasma composition is a critical factor in achieving selective inactivation of bacteria.
- Optimized plasma conditions can lead to enhanced bacterial killing with reduced impact on eukaryotic cells.
- This research supports the development of targeted plasma-based therapies for wound healing and infection control.
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