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Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
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Hypoxia increases epithelial permeability in human nasal epithelia
Hyun Jin Min1, Tae Hoon Kim2, Joo-Heon Yoon3
1Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.; The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, Korea.
Yonsei Medical Journal
|April 4, 2015
Summary
Hypoxia impairs nasal epithelial barrier function by decreasing junction proteins like ZO-1 and E-cadherin, increasing permeability. This barrier dysfunction may impact innate immunity against pathogens.
Area of Science:
- Cell Biology
- Immunology
- Respiratory Medicine
Background:
- The nasal mucosa acts as a critical barrier against pathogens.
- Nasal sinus inflammation is often associated with hypoxic conditions.
- Epithelial barrier integrity is vital for innate defense mechanisms.
Purpose of the Study:
- To investigate the impact of hypoxia on the barrier function of normal human nasal epithelial (NHNE) cells.
- To examine the effect of hypoxia on junction complex proteins in NHNE cells and human nasal mucosa.
Main Methods:
- Real-time PCR, Western blotting, and immunofluorescence assays were used to assess ZO-1 and E-cadherin expression.
- Trans-epithelial resistance (TER) was measured to evaluate changes in NHNE cell permeability under hypoxia.
- Protein levels of ZO-1 and E-cadherin were analyzed in human nasal mucosa samples.
Main Results:
- Hypoxia significantly decreased the mRNA and protein expression of ZO-1 and E-cadherin in NHNE cells.
- A time-dependent reduction in ZO-1 and E-cadherin was observed under hypoxic conditions.
- Hypoxia led to decreased TER in NHNE cells, indicating increased epithelial permeability.
- Human nasal mucosa samples exhibited lower levels of ZO-1 and E-cadherin compared to controls.
Conclusions:
- Hypoxia disrupts the expression of junction complex molecules in human nasal epithelia.
- The study demonstrates that hypoxia compromises epithelial barrier function, increasing permeability.
- This hypoxia-induced barrier dysfunction may contribute to innate immune dysfunction in the nasal cavity.
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